Spectrin and calmodulin in spreading mouse blastomeres
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چکیده
منابع مشابه
Spectrin and calmodulin in spreading mouse blastomeres.
The role of spectrin and its association with calmodulin in spreading mouse blastomeres was investigated. Embryonic spectrin binds 125I-calmodulin in a calcium-dependent fashion in the blot overlay technique. Double-labeling experiments show coordinate redistribution of spectrin and calmodulin in blastomeres preparing to undergo active spreading movement. At this stage cortical spectrin stainin...
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The relationship between myosin organization and cell spreading in the preimplantation mouse embryo was studied by indirect immunofluorescence in embryos cultured on lectin-coated substrates. Binding of cell surface polysaccharides to substrate-bound concanavalin A and wheat germ agglutinin induced changes in myosin distribution that resembled those which occur during cell-cell contact interact...
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The relationship between myosin organization and cell spreading in the preimplantation mouse embryo was studied by indirect immunofluorescence in embryos cultured on lectin-coated substrates. Binding of cell surface polysaccharides to substrate-bound concanavalin A and wheat germ agglutinin induced changes in myosin distribution that resembled those which occur during cell-cell contact interact...
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Calmodulin regulated spectrin-associated protein 1 (CAMSAP1) is a vertebrate microtubule-binding protein, and a representative of a family of cytoskeletal proteins that arose with animals. We reported previously that the central region of the protein, which contains no recognized functional domain, inhibited neurite outgrowth when over-expressed in PC12 cells [Baines et al., Mol. Biol. Evol. 26...
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Single blastomeres from 8-cell embryos adhere to lectin-coated agarose beads. The cells spread over the surface of peanut lectin (PNA), wheat germ lectin (WGA) and concanavalin A (Con A)-coated agarose beads, and this was prevented by sugars with high affinity for the appropriate lectin. Spreading was also prevented by cytochalasin D at a concentration (0.5-1.0 microgram/ml) known to inhibit co...
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ژورنال
عنوان ژورنال: Developmental Biology
سال: 1988
ISSN: 0012-1606
DOI: 10.1016/0012-1606(88)90237-0